Transfection is a process by which exogenous nucleic acids are delivered into cells to modify the genetic composition of host cells. It is widely used in the study of cellular processes and molecular mechanisms of diseases and in the development of gene therapies. Different types of nucleic acids, including deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs) and small non-coding RNAs (such as siRNA, shRNA and miRNA), can be transfected into mammalian cells by current transfection techniques.

Using liposomal or multicomponent transfection reagents is a well-established transfection method for delivery of nucleic acids into mammalian cell lines. This approach has been widely used for overexpression of proteins with expression plasmids or for specific gene knockdown using siRNA. However, many transfection reagents exhibit toxic side effects, such as reduced cell viability, and these side effects may lead to misinterpretation of the results. Therefore, selection of suitable transfection reagents is crucial to achieve valuable results with minimal side effects.

FuGENE® can be used in the presence or absence of serum, and their minimal cytotoxicity eliminates the need for medium changes after transfection. Compared with other methods, transfection of nucleic acids into mammalian cells using FuGENE® can achieve more consistent results, save time and effort, and has minimal impact on cell physiology.

FuGENE® DNA SiRNA Transfection Reagents

Delivery of DNA of all sizes into eukaryotic and insect cells requires transfection reagents that result in robust gene expression of the delivered construct with minimal-to-no cytotoxicity. Amerigo Scientific offers multi-component, non-liposomal or lipid-based transfection reagents with highly efficient transfection and extremely low cytotoxicity to meet the needs of diverse research, such as protein/antibody production, virus production, or breakthrough therapeutic development. Our FuGENE® DNA transfection reagents are suitable for both routine cost-effective transfection and transfection of sensitive, difficult-to-transfect primary or stem cells.